Peach latent mosaic viroid (PLMVd) occurs naturally in peach and nectarines worldwide including North and South America, Europe, Asia and Australia with high infection frequencies. Here, our experimental findings revealed for the first time PLMVd infections in walnut (Juglans regia L.) in the world. Twelve walnut leaves, all from symptomless plants, were obtained from a nearby walnut germplasm collection in Malatya (Turkey)