In the study, the effect of different doses of ultraviolet-B (UV-B) irradiation on the biochemical quality of Eregli Black Carrot (Daucus carotaL. spp. sativusvar. atrorubensAlef.) investigated, during storage. For this purpose, thecarrot roots treatedwith 0 kJm-² (control), 1.575 kJm-² (15m), 3.15 kJm-² (30m) and 6.30 kJm-² (60m) doses of UV-B were placed into the 1 kg sized PE bag and then stored in a cold room at 4 ± 1oC temperature and 85-90% relative humidity for 5 months. In the present study, it is found that even if UV-B applications increased the amount of total soluble phenol content (TSP) of carrots 1.16-1.53 fold during the first month of the experiment, later the amount of TSP decreased. During the storage period, the amount of TSP content changed between 23.72 and 51.11 mg CAE100 mL-1. While the lowest amount of anthocyanin has obtained from the control application with 557.76 mgkg-1FW, the highest value measured in UV-B 30m with 1636.96 mgkg-1FW. As a result of the study, it has determined that UV-B radiation of 3.15 kJ m-² dose could be used to increase the anthocyanin content of black carrots.